There are a number of things that could cause this.
Machine being out of squire, To test for this you just put a pencil in the collet and draw a 3,4,5 squire If the start and end points dont meat it's out of squire.
The ghost offset bug, What is just type G40 and G49 into the mdi to clear it.
The steps per being wrong, What's just measuring the axis movement if you tall it to move 8 inches does it move 8 inches.
Mach3 crapped out, A reinstall fixes this.
RF and EMF interferences, from the VFD, dust extractor (also includes static) or a outside sources. To test for this have the machine off, put a FM radio on the machine tune it into a radio chanell, then turn the machine on, if the radio goes all staticy, you have a RF or EMF leak.
To test for static put your hand on the machine's frame when it's cutting then go touch a bear hunk of metal or a person if you or the person get a zap , you have a static problem.
That's all the simple stuff, what takes minutes to check for if you do one at a time.
The acc or velocity being to high.
This is a Hard one that is easier to test with just the X motor turned on, you can just turn Y, Z and Y slave motor of in ports and pins.
Then run a engrave toolpath with about 20 letters if the start point and end point are the same the X axis is tuned fine, if they are not that's where the problem is if you have ticked off everything else.
Doing this test Mach3 has to do a lot of small moves and this is where Mach3 can fail, if it fails the acc or velocity needs tuned, if not it's something else like fusion being a ****.
Also you can just test once if it's going to fail it will on one run.
@supernetdude post a Gcode that has failed on the machine, You have posted your model so a comprar can be done on the G code in sim, then if I have time tomorrow I could test it on the big machine, what I have dialed in to 0.002 +/- .0001 per 1000 moves per axis.