Hi @sadibhuiyanbd,
The body passing through can be explained from the way the Rough contact was set up. Using the model from the previous post, there's a gap between the bodies which I believe is causing the contact to not take effect.
The Linear Static Stress study type assumes there are small displacements, and little to no dynamic movement of bodies. The contact uses an Activation Distance to determine if contact elements need to be generated before the solve (the contact elements are not shown in the UI). Since the gap is "large" the solver didn't create the needed contact elements automatically, which is causing the body to pass through.
This can be remedied by setting a Max. Activation Distance, so the solver knows to create contact elements before the solve to "span the gap" (a technical solution article about setting the Max AD can be found here).
A 2 mm Max AD was chosen by measuring the gap distance from the tip of root tine to the cylinder's face. Also, I removed some of the faces in the selection set that won't be involved in the contact.


The result shows the contact taking effect. However, it isn't real-life since the contacts elements are stiff enough to not allow movement to close the gap.

A better approach is to move the bodies close together initially, so they're almost touching. This way, the original Rough contact will take effect. I've done this by cloning the Simulation Model in the Simplify workspace, and moving the organic body 1.04mm closer to the cylindrical body (model attached).

Hope this helps.
Best regards,
Hugh Henderson
QA Engineer (Fusion Simulation)