Firstly I think you are now getting a floor loss because the floor is adjacent to another space and is not in direct contact with the ground or external environment. So its still not properly calculating floor loss for a ground floor.
Secondly I don't think you can do a simplistic comparison using the manual steady-state equation against the heat balance methodology used in the analysis software. You're also relying on your guesstimate of a temperature of 5C in the void but what if its actually 4C or 6C?
Thirdly, and very importantly, heatloss from ground floors does not in any case follow the simple Q=UAdeltaT equation because most of the loss is horizontally through the perimeter of the floor (if in direct contact with the ground) or the perimeter of the void (if the floor is suspended). It is fundamentally different from loss through a wall, window or roof which is in direct contact with outside air over all of its exposed area. Manual methods require a modified U-value to be used taking account of the perimeter to area ratio and loss through a floor of a given area will always be less than for a wall of the same area for that reason.
That said a lot has been written on this forum about the heat load and energy analysis calculations in Revit most of it not in a positive sense.
Do some searching and see what has been discussed before.
Revit uses the US Dept of Energy approved EnergyPlus software as its calculation engine, however that software is only as good as the data passed to it by Revit and having trawled through the various documents on the EnergyPlus website some years ago I came to the conclusion that EP is very comprehensive and should be as accurate as you're likely to get (in fact I think several expensive commercial applications use this freely downloadable software as their calculation engine). In my opinion therefore the problems lie with the way that Revit collates data to send to EP and the results which it then extracts from EP.
You could do a comparative test by exporting gbxml data from Revit and then importing it into the full version of EP rather than using the built-in command line interface version, however users have also queried the gbxml data which is output from Revit so this test is perhaps not 100% reliable either.
You could also as a comparative test (if I remember correctly) manually input data to EP so that it is processing raw data and not reliant on the output from Revit.
Hope this helps.