I’m not an expert on the DXF format, but I think what’s happening in your case is the following:
DXF is a unit-less format, which means it doesn’t store which measurement system you used to create it (mm, inches, pixels etc), just the numbers.
This means that if your laser cutter is importing the file without specifying the units that you used in creating it then it could scale incorrectly. In your case it looks like the importing program was set to inches so it read your 100x100 as inches, which would translate to 2540x2540mm.
The way to ensure this doesn’t happen is for you to import the file into, say, Adobe Illustrator (not the 2021 version, which has a scaling bug). In Illustrator the ‘Place’ option under the file menu has import options which you see by checking the box at the bottom of the Place sub-menu. This gives you options for selecting the units and the scaling factor. So in your case you would select ‘Original size’ and 1 to 1 in mm.
Save the file as a native illustrator file, or better still a PDF, then the laser software should see the size you intended.