I think what you're encountering is this (pardon if I do a little mansplaining 🙂 )
When you do "post processing", Fusion will run your CAM setup through a, well, "post processor" which is a script file (is it in the Python language? I can't remember). This is basically a program script (interpreted language, as opposed to a compiled language such a C++, Java, etc) that reads your CAM configurations and generates your GCode files.
The default post processor, which I suspect is the one you edited before, was probably recently overwritten by an update to Fusion. This is not a bug, it's how it's designed to work. If you want to customize the script, you must make a copy and save it someplace on your computer (or in the cloud as @daniel_lyall ssuggested). That moves it out of the location where Fusion is assuming it has the right to make updates). You then modify your copy, and the select that copy as your post processor, and it won't get override by software updates, which are now happening automatically even if you don't want them. 🙂
See attached screen capture, note that I have selected "personal posts" as the source for my PP file, and then I selected the one named "Jarvis_...." (because Iron Man rocks). That is simply what I renamed the copied file too. On my system, which is Mac OS, the file resides in ~user/Autodesk/Fusion 360 CAM/posts. Yours may be different if you're on Windoze, etc. Yes, it's a PIA research to figure that out.
Now, the downside of all this, is that if Autodesk makes a GOOD update to the post, you will miss out on that. And of course, they don't let you know that it has changed. Might be worth making notes as to what you've changed, and just periodically checking the version of the default one, then re-editing it to make your changes. What I would prefer to see if some sort of "post processing" hook for the post processor (pardon the overuse of terminology), where I could put in a little bit of script to say "remove this", or better yet, the ability to override JUST the one method that is adding that G53, so that I would still get the rest of the post updates.
Hope this helps.
