Of course if you know in advance that the hole will be used in the assembly ...
Just wondering, what scenario would that be where your putting a hole into a part that you don't know wether it will be used in the assembly?
You are right in most cases the hole is used by a screw. :-)
Let me suggest another idea. If you know in advance that the patterned hole will be used by a screw, I suggest to attach/mark the 1st hole with iMate (Insert type). In most cases the screw has iMate at the "correct" location or you can edit the screw part and add a similar iMate (Insert type) to the screw. In the assembly you can create a constraint/iMate result which will put together the hole with the screw. In this case we have more steps and this workflow is good when you have more pieces of the part with patterned hole.
In the pattern dialog box. If you hit the rectangular botton you can select the alignment in both directions.
I think you may be lost...